| Title | Actinocrinis puniceicyclus gen. nov., sp. nov., an actinobacterium isolated from an acidic spring |
| Author | Kim, J -J; Marjerrison, C E; Cornish Shartau, S L; Brady, A L; Sharp, C E; Rijpstra, W I C; Sinninghe Damsté, J S; Schumann, P; Grasby, S E; Dunfield, P F |
| Source | International Journal of Systematic and Evolutionary Microbiology vol. 67, issue 3, 2017 p. 602-609, https://doi.org/10.1099/ijsem.0.001667 (Open
Access) |
| Year | 2017 |
| Alt Series | Natural Resources Canada, Contribution Series 20170013 |
| Publisher | Microbiology Society |
| Document | serial |
| Lang. | English |
| Media | paper; on-line; digital |
| File format | pdf; html |
| Subjects | bacteria; taxonomy; nomenclature; wetlands; bogs; Actinobacteria; Actinomycetales; Catenulisporineae; Actinospicaceae; Actinospica robiniae; Actinocrinis puniceicyclus gen. nov., sp. nov.; microbiology;
genetics; rRNA |
| Illustrations | photomicrographs; graphs; tables; biostratigraphic charts |
| Program | Energy Geoscience, Geothermal Assessments |
| Program | NSERC Natural Sciences and Engineering Research Council of
Canada |
| Released | 2017 04 03 |
| Abstract | An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada. Cells of isolate OB1(T) were Gram-stain-positive, non-motile, pink- to
purple-pigmented filaments. The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30ºC. The major cellular fatty acids were i-C(16:0) (28.5 %), i-C1(5:0) (14.6 %) and ai-C1(5:0) (14.3 %), and the major polar lipid
was phosphohexose. The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1-gamma. The DNA G+C content was 70.2 %. Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy
broth, growth occurred on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate). Anaerobic growth was not observed. The cells did not fix atmospheric nitrogen. On the basis
of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales. The most closely related species was Actinospica robiniae. However, the 16S rRNA gene
sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species. We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp. nov., with strain OB1(T) (=DSM
45618(T)=ATCC BAA-2771(T)) as the type strain. |
| Summary | (Plain Language Summary, not published)
This work examines microbial communities found in thermal springs systems being studied in relationship to geothermal potential. In this case a unique
DNA sequence was found and it is argued to be a newly discovered genus of microbial life. |
| GEOSCAN ID | 300527 |
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