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TitleActinocrinis puniceicyclus gen. nov., sp. nov., an actinobacterium isolated from an acidic spring
AuthorKim, J -J; Marjerrison, C E; Cornish Shartau, S L; Brady, A L; Sharp, C E; Rijpstra, W I C; Sinninghe Damsté, J S; Schumann, P; Grasby, S E; Dunfield, P F
SourceInternational Journal of Systematic and Evolutionary Microbiology vol. 67, issue 3, 2017 p. 602-609, (Open Access)
Alt SeriesNatural Resources Canada, Contribution Series 20170013
PublisherMicrobiology Society
Mediapaper; on-line; digital
File formatpdf; html
Subjectsbacteria; taxonomy; nomenclature; wetlands; bogs; Actinobacteria; Actinomycetales; Catenulisporineae; Actinospicaceae; Actinospica robiniae; Actinocrinis puniceicyclus gen. nov., sp. nov.; microbiology; genetics; rRNA
Illustrationsphotomicrographs; graphs; tables; biostratigraphic charts
ProgramEnergy Geoscience, Geothermal Assessments
ProgramNSERC Natural Sciences and Engineering Research Council of Canada
Released2017 04 03
AbstractAn aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada. Cells of isolate OB1(T) were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments. The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30ºC. The major cellular fatty acids were i-C(16:0) (28.5 %), i-C1(5:0) (14.6 %) and ai-C1(5:0) (14.3 %), and the major polar lipid was phosphohexose. The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1-gamma. The DNA G+C content was 70.2 %. Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occurred on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate). Anaerobic growth was not observed. The cells did not fix atmospheric nitrogen. On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales. The most closely related species was Actinospica robiniae. However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species. We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp. nov., with strain OB1(T) (=DSM 45618(T)=ATCC BAA-2771(T)) as the type strain.
Summary(Plain Language Summary, not published)
This work examines microbial communities found in thermal springs systems being studied in relationship to geothermal potential. In this case a unique DNA sequence was found and it is argued to be a newly discovered genus of microbial life.