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TitleGlobal metagenomic survey reveals a new bacterial candidate phylum in geothermal springs
AuthorEloe Fadrosh, E A; Paez Espino, D; Jarett, J; Dunfield, P F; Hedlund, B P; Dekas, A E; Grasby, S E; Brady, A L; Dong, H; Briggs, B R; Li, W; Goudeau, D; Malmstrom, R; Pati, A; PettRidge, J; Rubin, E M; Woyke, T
SourceNature Communications; Nature Communications 2016 p. 1-10, https://doi.org/10.1038/ncomms10476
Year2016
Alt SeriesEarth Sciences Sector, Contribution Series 20150379
PublisherNature
Documentserial
Lang.English
Mediapaper; on-line; digital
File formatpdf
ProvinceBritish Columbia
NTS82F/09
AreaDewar Creek; Tengchong; Great boiling spring nevada; United States
Lat/Long WENS-116.5000 -116.0000 49.7500 49.5000
Lat/Long WENS 98.0000 99.0000 25.5000 25.0000
Lat/Long WENS-119.5000 -119.0000 40.7500 40.5000
Subjectsthermal springs; microorganisms; bacteria; metagenomic data; novel bacterial phylum; genome reconstruction
Illustrationsdiagrams; photographs; photomicrographs; location maps; structural diagram; charts
Programenvironmental impacts and adaptation in the northern environment, Environmental Geoscience
AbstractAnalysis of the increasing wealth of metagenomic data collected from diverse environments can lead to the discovery of novel branches on the tree of life. Here we analyse 5.2 Tb of metagenomic data collected globally to discover a novel bacterial phylum ('Candidatus Kryptonia') found exclusively in high-temperature pH-neutral geothermal springs. This lineage had remained hidden as a taxonomic 'blind spot' because of mismatches in the primers commonly used for ribosomal gene surveys. Genome reconstruction from metagenomic data combined with single-cell genomics results in several high-quality genomes representing four genera from the new phylum. Metabolic reconstruction indicates a heterotrophic lifestyle with conspicuous nutritional deficiencies, suggesting the need for metabolic complementarity with other microbes. Co-occurrence patterns identifies a number of putative partners, including an uncultured Armatimonadetes lineage. The discovery of Kryptonia within previously studied geothermal springs underscores the importance of globally sampled metagenomic data in detection of microbial novelty, and highlights the extraordinary diversity of microbial life still awaiting discovery.
Summary(Plain Language Summary, not published)
Microbial communities play critical roles in geologic processes, including mobilization and retention of toxic metals, and generation of natural gas in shallow aquifers. Close work between geochemists and microbiologists is making strides in understanding these processes. Recently it has been recognized that traditional methods for identification of microbes has 'blind spots' and unusual microbial life is missed. New methods using metagenomic studies, combined with microbial samples collected from extreme environments as part of previous NRCan geothermal research, has led to discovery of a new bacterial phylum (equivalent to a new Kingdom of life for higher organisms). This remarkable discovery provides new knowledge on the diversity of life on Earth. Lessons from this work will be applied to the Environmental Geoscience Program to aid study of novel microbial communities that can help limit metal mobility in contaminated environments.
GEOSCAN ID297488